Genetic Characterization of Diethylnitrosamine-induced Purple Adenine (ad-3) Mutants in Neurospora crassa1

Genetic characterization of diethylnitrosamine-induced purple adenine (ad-3) mutants in Neurospora crassa.

conditions was the compound found to be mutagenic. Mutants were induced in a gentically marked 2-component heterokaryon and recovered as purple colonies among the white, nonmutant background colonies. In this system, purple colonies result from two types of genetic damage in the adenine-3 region: point mutations at ad-3A and ad-3B loci and multilocus deletions covering one or both loci simultan...

Studies with Purple Adenine Mutants in Neurospora Crassa. III. Reversion of X-Ray-Induced Mutants.

Studies with Purple Adenine Mutants in Neurospora Crassa. IV. Lack of Complementation between Different Ad-3a Mutants in Heterokaryons and Pseudowild Types.

1 N Neurospora a useful method of screening for alleles in a series of mutants with identical biochemical requirements is the test for complementation in heterokaryons. Mutants that complement in such tests and enable the heterokaryon to grow on minimal medium are considered nonallelic and are assumed to result from mutation at different genetic loci; whereas mutants that do not complement are ...

Enzymology of the pigmented adenine-requiring mutants of Saccharomyces and Schizosaccharomyces.

The red or purple adenine-requiring mutants of Saccharomyces, Schizosaccharomyces, and Neurospora have been categorized genetically as resulting from the mutation of two separate loci. In Neurospora crassa these are the ad-3A and ad-3B loci (de Serres, 1956); in Saccharomyces cerevisiae, the ad-l and ad-2 loci (Roman, 1956); and in Schizosaccharomyces pombe, the ad-6 and ad-7 loci (Leupold & Gu...

Mutation induction by difunctional alkylating agents in Neurospora crassa.

The genetic characterization of ad-3 mutants of Neurospora crassa induced by two carcinogenic difunctional akylating agents, 1,2,4,5-diepoxypentane (DEP) and 1,2,7,8-diepoxyoctane (DEO), has shown that point mutations at the ad-3B locus have similar complementation patterns. In addition to the induction of point mutations, DEP induces a low frequency (7.5%) of multilocus deletions, whereas DEO ...